The impact of cross-reactivity of the various swine
mycoplasmas on the various commercial M hyopneumoniae
ELISA assays is not well characterized. Our results
indicate that cross-reactivity can occur with the IDEXX
ELISA, which showed positive responses in a few animals
from both M. hyosynoviae and M. flocculare-challenged
groups (Tables S2 and S3). The M. hyopneumoniae Tween
20 assay did not show cross-reactivity against the sera
from the other three challenge groups (Figs. S2–S4). This
may be due to its lower sensitivity of the latter.