4.1. Effect of Concentration of Polysaccharides on RS3 Formation
The RS3 formation was dependent on polysaccharide concentration. The RS3 content kept increasing with the gum concentration rising from 0% to 0.2%, but at higher concentrations, a decrease was observed. This trend indicated that with the increasing concentration of polysaccharides from 0% to 0.2%, the reaction between starch and gum facilitated RS3 formation, however, when the concentration was over 0.2%, the solution viscosity was too high for RS formation.
It could be observed from Figure 1 that the effects of the three gums on RS3 formation were different. For concentrations of polysaccharides lower than 0.1%, the RS3 content did not change remarkably among the three polysaccharides, but all increased slightly. RS3 content with the presence of KGM increased more significantly compared with the other two samples, while the polysaccharide concentrations were between 0.1% and 0.2%. The addition of CA had the smallest effect on RS3 formation. Furthermore, there was a sharp decrease of RS3 content with the presence of KGM within polysaccharide concentration from 0.2% to 0.3%, while a slow decrease with the presence of the other two gums. When the concentration of polysaccharides was more than 0.3%, no remarkable change of RS3 content was observed.
The interaction between polymers is a very complex process, which could be influenced by a range of factors. With other conditions (temperature, pH, etc.) being kept equal, the different characteristics of the gums co-existing with the starch could be employed to explain the results. According to the former research of the same team [16], RS3 content was bound up with the properties of the starch-gum gel. During storage, the lower the viscosity of the gel, the more violent was the molecular motion. Violent molecular motion was in favor of the formation of a more compact double helix structure. Contrariwise, high viscosity blocked the molecular motion of the gel, and resulted in less crystal nucleus. As has been found with increasing KGM concentration, the adhesiveness of the mixed gel did not change significantly [16]; thus, the presence of KGM boosts the leaching of amylose during gelatinization. In addition, the molecular motion of amylose facilitates the formation of a crystal nucleus during storage of the starch-gum mixture.
4.1. Effect of Concentration of Polysaccharides on RS3 Formation
The RS3 formation was dependent on polysaccharide concentration. The RS3 content kept increasing with the gum concentration rising from 0% to 0.2%, but at higher concentrations, a decrease was observed. This trend indicated that with the increasing concentration of polysaccharides from 0% to 0.2%, the reaction between starch and gum facilitated RS3 formation, however, when the concentration was over 0.2%, the solution viscosity was too high for RS formation.
It could be observed from Figure 1 that the effects of the three gums on RS3 formation were different. For concentrations of polysaccharides lower than 0.1%, the RS3 content did not change remarkably among the three polysaccharides, but all increased slightly. RS3 content with the presence of KGM increased more significantly compared with the other two samples, while the polysaccharide concentrations were between 0.1% and 0.2%. The addition of CA had the smallest effect on RS3 formation. Furthermore, there was a sharp decrease of RS3 content with the presence of KGM within polysaccharide concentration from 0.2% to 0.3%, while a slow decrease with the presence of the other two gums. When the concentration of polysaccharides was more than 0.3%, no remarkable change of RS3 content was observed.
The interaction between polymers is a very complex process, which could be influenced by a range of factors. With other conditions (temperature, pH, etc.) being kept equal, the different characteristics of the gums co-existing with the starch could be employed to explain the results. According to the former research of the same team [16], RS3 content was bound up with the properties of the starch-gum gel. During storage, the lower the viscosity of the gel, the more violent was the molecular motion. Violent molecular motion was in favor of the formation of a more compact double helix structure. Contrariwise, high viscosity blocked the molecular motion of the gel, and resulted in less crystal nucleus. As has been found with increasing KGM concentration, the adhesiveness of the mixed gel did not change significantly [16]; thus, the presence of KGM boosts the leaching of amylose during gelatinization. In addition, the molecular motion of amylose facilitates the formation of a crystal nucleus during storage of the starch-gum mixture.
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