Real-time PCR is an ideal technique for ecological studies as a
single DNA extract can be analyzed for many genes, allowing a
quantitative approach to analyzing both community structure
and function. In this study, we found that Real-time PCR
was more reproducible, less labor-intensive, and quicker techniques
for the study of dynamic of the microbial community in
the contaminated seawater mesocosms and data obtained
revealed that bioremediation actions (biostimulation and bioaugmentation)
stimulated crude oil degrading bacteria than
other genus in the marine microbial community. However, it is
important that Real-time PCR assays are tested thoroughly for
specificity and reproducibility and that the limitations of the
method are recognized.