Quantification of medicinal substance using spectrophotometer
may carried out by preparing solution in transparent solvent and
measuring it’s absorbance at suitable wavelength. The wavelength
normally selected is wavelength of maximum absorption (λmax),
where small error in setting the wavelength scale has little effect on
measured absorbance. Ideally, concentration should be adjusted to give
an absorbance of approximately 0.9, around which the accuracy and
precision of the measurements are optimal.