One milliliter of this solution was added to 1 mL of 2% AlCl3 dissolved in methanol and 3 mL of 5% sodium acetate in methanol. After incuba-
tion at room temperature for 2 h 30 min, the absorbance was measured
at 440 nm. Rutin was used as standard flavonol. The flavonol content
was expressed as mg of rutin equivalents per 100 g DW. The experi-ments were always performed in triplicate.