2.2 Cell counting
Spread plate method used for counting the number of cells in yogurt (Aquirre and Collins, 1993)
2.2.1 Pipette yogurt 1 ml. Dilute with 9 ml of distilled water, and make serial dilution.
2.2.2 Pipette 0.1 ml of the dilution to culture medium, Lactobacillus bulgaricus agar and Streptococcus
thermophilus agar (Orihara, Sakauchi and Nakazawa, 1992)
2.2.3 Dip triangle glass rod in alcohol, then fire and wait to cool. Use glass rod to spread dilution on surface of
medium until dry.
2.2.4 Incubate medium at 40-45 ° C for 48 hours in anaerobic jar which have Anaerocult ® A. Count bacterial
colonies.