(7) Wait for samples to reach room temperature and add 300 mL of saturated NaCl. Centrifuge at 10,000 g at 4 8C for
10 min.
(8) Transfer the supernatant (Fig. 1e) to another 2 mL tube, add 160 mL of 5 M NaCl and homogenize.
(9) Add 600 mL of CIA (chloroform and isoamyl alcohol 24:1) and centrifuge at 10,000 g at 4 8C for 10 min