Aliquots of aqueous methanol phenolic extract (free and bound)
of the sample was mixed with DPPHþ reagent and absorbance was
read at 516 nm as outlined by Yen and Chen (1995). The percentage
of scavenging effect was calculated by using the following equation:
DPPH radical scavenging capacity (%) ¼ [1 (A516 of sample/A516
of blank)] 100. Results were expressed as mg Trolox equivalents
per 100 g of sample on a dry weight basis (mg TE/100 g dw).