Thus, the biosensor displayed good selectivity for the determination
of β-lactamase.
4. Conclusions
A straightforward and sensitive ECL biosensor was developed
for the detection of β-lactamase based on the novel recognition
element of Ru-Amp, which was one of hydrolyzed substrates of
the β-lactamase. A novel Ru-Amp complex here acted not only as
ECL reagent for the ECL emitters but also as specific recognition
element of β-lactamase. And Ru-Amp/TA@AuNPs nanomaterials
generated a very stable film, which exhibited excellent ECL behaviors.
On the basis of their excellent performance, this biosensor
for the detection of β-lactamase achieved a detection limit of
37 pg mL1 and exhibited excellent selectivity. The simple ECL
tactics would be promising in applications of biotechnology and
clinical diagnosis.