In cases in which the intrinsic fluorescence of a test compound
overlapped with the fluorescence of the reaction product, the substrate MFC was substituted by dibenzylfluorescein (DBF) which
was converted by aromatase to fluorescein (kex = 485 nm;
kem = 530 nm).41 The protocol was the same as the one above, except
for a longer incubation time (2 h) and the use of a different
stop reagent (2 M NaOH).