Micro dilution assay
The minimal inhibition concentration (MIC) values were determined for the bacteria determined as sensitive to the EO in disc diffusion assay. The inoculate of bacteria were prepared from 12 h broth cultures and cultures were adjusted to 0.5 McFarland Standard turbidity. The EO
concentrations were prepared between 500 μl/ml to 7.81 μl/ml and tested in 10 ml sterile test tubes con-