This project aims to examine microbial contamination on equipment used in the diet such as plates on the spoon on the table on the ladle and on the area of the hand's chef. Our focus for E.coli . This will also cause contamination of pathogenic microorganism and to find way to prevent and resolve such cause in the future. The samples of bacteria on equipment used to eating these 5 types such as plates on the spoon on the table on the ladle and on the area of the hand's chef. And microorganisms from the samples. The cultured on agar PCA and incubated for 48 hours. Then count the number of colonies to determine the quantity of microorganisms. Which found that the area of the hand of chef is the maximum number of microorganisms as ladle ,plate, spoon and table , respectively. And select the colony look like E.coli. After that fed on EMB agar agar for 48 hours. Found that microorganisms are growing. But colony are supposed to be based on E.coli. The colony will be green. It was concluded that the bacteria were not the E.coli. The survey was conducted for those types of contamination on the equipment used to eating these 5 types. By focusing on microbes that cause diarrhea. The microorganisms to detect Biochemical Tests releta Gram staining , Catalase Tests , Triple sugar iron agar (TSI ) , Gelatin ligvefaction) and Motility test .
From the test results Staphylococcus aureus and Klebsiella bacteria, which is why the two types that cause diarrhea. So there are ways to prevent the two types of microorganism is the hob should be hygienic and wash hands before cooking and should dress appropriately for cooking is wearing an apron and wear a hat in cooking. Including students in the school, everyone should wash their hands before every meal to protect against the two types of bacteria and other species