At the first 2–6 days, the colonies of B. braunii 765 cultivated in the BG11 medium appeared looser structure than those of the inoculums, with a diameter of 15–20 lm vs. 10 lm. Then, the colony size gradually recovered and remained stable thereafter. By comparison, the compact degree of algal colonies in the aerated swine lagoon wastewater changed little at first. But after 6 days from inoculation, some clusters (30–50 lm in diameter) formed by 3–5 single colonies appeared in the wastewater and became more and more. The formation of such clusters might be related to an increase of the algal extracellular polysaccharides, whose production would be favored by the high C/N ration of the swine wastewater (Miqueleto et al., 2010). In addition, these bigger colonies of B. braunii 765 in the wastewater would make it easier to harvest the alga biomass by auto-flocculation.
At the first 2–6 days, the colonies of B. braunii 765 cultivated in the BG11 medium appeared looser structure than those of the inoculums, with a diameter of 15–20 lm vs. 10 lm. Then, the colony size gradually recovered and remained stable thereafter. By comparison, the compact degree of algal colonies in the aerated swine lagoon wastewater changed little at first. But after 6 days from inoculation, some clusters (30–50 lm in diameter) formed by 3–5 single colonies appeared in the wastewater and became more and more. The formation of such clusters might be related to an increase of the algal extracellular polysaccharides, whose production would be favored by the high C/N ration of the swine wastewater (Miqueleto et al., 2010). In addition, these bigger colonies of B. braunii 765 in the wastewater would make it easier to harvest the alga biomass by auto-flocculation.
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