2.8. Statistical analysis
All experiments were performed in triplicate and data were presented
as the mean ± standard deviation. The statistical analyses
were carried out using SPSS software (SPSS Statistics v21, IBM, UK). The
statistically significant differences among different variables were performed
using one-way analysis of variance (ANOVA) followed by Sidak's
Post Hoc Test. In all comparisons, p b 0.05 was considered statistically
significant.
3. Results and discussion
3.1. The effect of processing parameters
3.1.1. The concentration of crosslinking agent CaCl2
A series of CaCl2 crosslinking solutions, from 0.01 M to 0.5 M, were
used to collect electrosprayed GLS/A beads. At 0.01 M CaCl2, individual
spherical beads tended to agglomerate into lumps. When the CaCl2 concentration
increased to 0.05 M, individual GLS/A beads started to form.
By further increasing the concentration of CaCl2 to 0.5 M, the coagulation
phenomenon can be eliminated.
2.8. Statistical analysisAll experiments were performed in triplicate and data were presentedas the mean ± standard deviation. The statistical analyseswere carried out using SPSS software (SPSS Statistics v21, IBM, UK). Thestatistically significant differences among different variables were performedusing one-way analysis of variance (ANOVA) followed by Sidak'sPost Hoc Test. In all comparisons, p b 0.05 was considered statisticallysignificant.3. Results and discussion3.1. The effect of processing parameters3.1.1. The concentration of crosslinking agent CaCl2A series of CaCl2 crosslinking solutions, from 0.01 M to 0.5 M, wereused to collect electrosprayed GLS/A beads. At 0.01 M CaCl2, individualspherical beads tended to agglomerate into lumps. When the CaCl2 concentrationincreased to 0.05 M, individual GLS/A beads started to form.By further increasing the concentration of CaCl2 to 0.5 M, the coagulationphenomenon can be eliminated.
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