As indicated above, CT-IC can discriminate CT from LT
and did not react with nontoxigenic V. cholerae strains. The
specificity of CT-IC was further evaluated by examining the
culture of bacteria other than V. cholerae. In this study, we
examined the reactivity of CT-IC against cultures of ETEC
and V. parahaemolyticus. As mentioned above, ETEC strains
have the ability to express LT protein which shares high homology
with CT. V. parahaemolyticus is the most frequently
isolated species among genus Vibrio and is one of the most
important food-borne pathogen worldwide. In the “gold
standard” selective cultivation test, the same enrichment and
selective media are usually used for isolations of V. cholerae
and V. parahaemolyticus. Twelve lt gene-positive ETEC isolates
and 7 V. parahaemolyticus isolates were cultured under