Wheat bran, soybean meal and cottonseed meal, alone or in combinations, were used as the substrates
for simultaneous production of protease and -amylase by Aspergillus oryzae under solid state fermentation
using a simplex centroid mixture design. The substrates with highest water absorption index and
more homogeneous granulometric distribution have positively influenced on enzymes production. Synergistic
effects of agroindustrial wastes observed on the production of protease and -amylase resulted
in increases ranged from 6.3 to 106.1% at 24 h fermentation. Mixtures contained wheat bran (1/2) and
soybean meal (1/2) or wheat bran (1/2) and cottonseed meal (1/2) may be added to improve the enzymes
production in shorter time fermentation. However, at 48 and 72 h fermentation, the most of the interaction
effects observed were antagonistic and the highest protease and -amylase activities were obtained
wheat bran as the substrate. This innovative process showed maximization of -amylase and proteases
production when mixtures were used compared to the isolated substrates and can be extended to other
enzymes groups for obtaining multi-enzyme complex.
©