Results are interesting. Hot water wash is almost similar to enzyme treated. But, when decant starch after hot water treatment, we are also removing soluble fibers, which is very much similar to soluble starch on solubility.
You will find, if you dissolve both in hot water, enzyme treated solution will be hazy compared and hot water extracted will be clear top layer.
I shall bring a chemical called Anthrone and Cuvettes for the Spectrophotometer; you will start the starch analysis using the Spectronic-20. Request Mr Ding to check the both the Precision Expro weighing scale and Spectronic-20 kept in the Khun Sinchai's meeting room.
The objective is to keep the starch less than1% without removing soluble fibers.
I noticed another point after referring the Product Specification of the enzymes that it will react at high temp 80-90C. Another variant of the enzyme (mostly) will have optimum reaction at 35-45C.
Hence, if possible conduct reaction in the same way what we did in the Univ, but keep the water bath temp at 80-85C. I believe the enzyme is stored in the University. Let us get 50%, and freeze as per the Product Data Sheet and advise them to store in the way. I shall also send a power sample alpha amylase, which will work at 35-37C.