According to literatures, the extra

According to literatures, the extraction of sex
hormones from antler velvet is accomplished by vibrating,
heating, or refluxing. For example, estradiol
was extracted by hot refluxing method with methanol
solvent [13]. Vibration extraction with acetone and
methanol mixture solution was adopted to obtain sex
hormone components [14], and then solid-liquid extraction
with chloroform was performed for further
HPLC analysis. Moreover, 90% methanol solution or
pure dichloromethane and KOH (0.1 mol·L