The aim of preparation is to extract concentrated pollens and spores in the sediment by removing extraneous matter both mineral and organic matter from the samples. It has been known that there is no standard set of treatment to prepare palynological samples; a better understanding of the potential and limitation of different treatments and components of the residues can maximize productivity of palynomorph.
Though the recommended quantity of soil material for pollen extraction is small amount (19), the amount of soil and chemical treatments can be adapted suitably. After each step in chemical processes, centrifuging samples at 2500 r.p.m. for 3 minutes is preferred. For preparation processes, the consideration of safety is so much important because many dangerous chemicals are use in chemical processes such as HCl, HF and Conc. H2SO4. Steps of processes are followed the standard techniques of many authors