For this purpose 4μl of genomic
DNA was used as template, 2μl of 10X PCR
buffer (Fermentas cat# B34), 2μl of 2mM
dNTPs, 1μl of MgCl2 (Fermentas cat#
R0971), 2μl of 10 p-mol forward primer (5’
TCTTTGCAGGTCAAGGCTCT 3’), 2μl of
10 pmol reverse primer (5’
ATTTGCTCCCACGATGAAAC 3’)