In 1991, the first regenerable oil palm embryogenic cell suspension cultures were obtained from Oil palm leaf-derived calli( de Touchet et al.,1991) Later, mature zygotic embryo excised from fruits were used to generate friable embryogenic tissue and to establish embryogenic. Although these two protocols were successful for estabilishing single somatic embryos from embryogenic suspension culture, the regeneration rate into plantlets was poor. This issue was overcome by Aberlenc-Bertossi et al., (1999), who developed a protocol to improve the quality of shoot formation (Gorret et al., 2004).