Fig. 4. The effects of FBS concentration and individual fatty acid supplementation on Δ6
Fads (filled columns) and Elovl5 (cross-hatched columns) gene expression in the SBT-E1
cell line. In Panel A, the cells were incubated under the different conditions for 3 days
and the fatty acids were supplied at a concentration of 10 μM. In Panel B, the cells were
incubated under the different conditions for 24 h and the fatty acids were supplied at a
concentration of 10 μM. In Panel C, the cellsweremaintained in standard culture medium
containing 10% (v/v) FBS. In Panels A and B, the data have been normalised using β-actin
and are expressed relative to the control, arbitrarily set at a value of 1.0. In Panel C, the
transcript abundances forΔ6 Fads and Elovl5 are expressed relative to the transcript abundance
for β-actin. The data are themean±standard error of themean (n=3). Significant
differences between the treatments and the control are indicated by an asterisk.
Fig. 4. The effects of FBS concentration and individual fatty acid supplementation on Δ6Fads (filled columns) and Elovl5 (cross-hatched columns) gene expression in the SBT-E1cell line. In Panel A, the cells were incubated under the different conditions for 3 daysand the fatty acids were supplied at a concentration of 10 μM. In Panel B, the cells wereincubated under the different conditions for 24 h and the fatty acids were supplied at aconcentration of 10 μM. In Panel C, the cellsweremaintained in standard culture mediumcontaining 10% (v/v) FBS. In Panels A and B, the data have been normalised using β-actinand are expressed relative to the control, arbitrarily set at a value of 1.0. In Panel C, thetranscript abundances forΔ6 Fads and Elovl5 are expressed relative to the transcript abundancefor β-actin. The data are themean±standard error of themean (n=3). Significantdifferences between the treatments and the control are indicated by an asterisk.
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