Fig. 1 shows the zones of hydrolysis obtained during the isolation process prior to selection of the highest-yielding strain. Selected colonies were round, smooth, raised and had a creamy to yellow appearance. Results obtained for the biochemical tests performed are as shown in Table 1. Initially thought to be a Pseudomonas species, further tests performed showed that the isolate also produced fluorescence under UV light on blood agar. In addition to this, successfully amplified DNA samples (Fig. 2) were sent for sequencing and analysis of the resulting 16S rDNA sequence revealed that the isolate was related to the genus Naxibacter, from the family of Oxalobacteraceae, with 99% homology. Pairwise alignment with different species of the genus showed a 98% sequence similarity with N. haematophilus
followed by N. varians (97.5%).
Fig. 1 shows the zones of hydrolysis obtained during the isolation process prior to selection of the highest-yielding strain. Selected colonies were round, smooth, raised and had a creamy to yellow appearance. Results obtained for the biochemical tests performed are as shown in Table 1. Initially thought to be a Pseudomonas species, further tests performed showed that the isolate also produced fluorescence under UV light on blood agar. In addition to this, successfully amplified DNA samples (Fig. 2) were sent for sequencing and analysis of the resulting 16S rDNA sequence revealed that the isolate was related to the genus Naxibacter, from the family of Oxalobacteraceae, with 99% homology. Pairwise alignment with different species of the genus showed a 98% sequence similarity with N. haematophilusfollowed by N. varians (97.5%).
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