What is more, the ratio of the absorbance (995/1022 cm1) provided a better way to characterize
the internal changes in double helix than that of the absorbance (1047/1022 cm1). Analysis of the IR spectrum indicated that the ratios of absorbance (995/1022 cm1) of native starch, NP-LRS3,
GP-LRS3, ZP-LRS3 and HAMS were 0.87, 1.01, 1.00, 1.01, and 0.97, respectively. The proportion of crystalline region in LRS was higher than in either native starch or HAMS, and this was confirmed
using X-ray diffraction