ne of the most important advantages of using im-mobilised cells is to increase the extent of growth andproduction period and repeated batch experimentswere, therefore, conducted. When maximal lipolyticactivity was recorded the used medium was replacedwith fresh medium, aseptically. While, initially, themaximum activity was measured after 18 h of fermenta-tion, after 120 h of fermentation this activity wasobtained within 2 h (Fig. 7). This result showed thatimmobilisedR.arrhizuscells might be kept viable andcould produce the enzyme for a 120 h period