In most of the extracts examined by TLC and UV
light, deep purple or violet colours appeared which indicate the presence of flavanones, flavones and flavonols.
These compounds are in the form of diglycosides as
demonstrated by the Rf values in the two-dimensional
TLC using hesperidin (flavanone diglycoside) as a standard. This is clear in the extracts obtained by ethyl acetate and n-butanol.