The research examined the antimicrobial properties of Russula nigricans and Phellinus sp. against six different strains of pathogenic bacteria: Escherichia coli, Pseudomonas aeruginosa, Proteus sp., Salmonella sp., Bacillus cereus, and Staphylococcus aureus. An extract was prepared from the fruiting bodies of these mushrooms using 70% ethanol and water. The evaluation of the growth inhibitory effect of each mushroom extract was carried out using the agar-well diffusion, minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The inhibitory effect of the crude extract obtained from 70% ethanol extract of R. nigricans toward B. cereus was found to be the most pronounced, as shown by the MIC and MBC values of 6.25 and 25 mg/ml, respectively. It was found that the water extract of R. nigricans exhibited the most potent activity against S. aureus as indicated by its MIC and MBC values of 50 mg/ml. Furthermore, B. cereus was inhibited by the 70% ethanol extract of Phellinus sp. at MIC and MBC values of 50 mg/ml. Phellinus sp. water extract inhibited B. cereus at 12.5 and 50 mg/ml MIC and MBC, respectively.