After development, the solvent
was removed by flushing the rods with a stream of nitrogen
and drying in an oven for 2–3 min before analyzing
with the flame ionization detector. The sample analyses
were carried out under the following conditions: flow
rate of hydrogen, 160 ml/min; flow rate of air,
2000 ml/min; scan speed, 30 s/scan. Peak area was quantified
and expressed as mg/g sample dry matter.