Preparation of the fermentation medium
The orange fruits (Citrus sinensis) were purchased at a local
market in Lagos. They were thoroughly washed with 0.1% of
sodium metabisulphite solution, cut into pieces and pressed
manually to obtain the juice. The juice was sterilized with 200 mg/L
sodium metabisulphite and allowed to clarify at –5°C for 24 h. The
supernatant was analysed for the total soluble solid and the pH.
The total soluble solid was fortified with sucrose from 12.5 to
18°Brix to give enough fermentable sugar.