Twomonth-
old in vitro tomato plants (three plants or replications per
treatment) were inoculated by applying Xcv cell suspension to
leaves using micropipettes. After inoculation, three plants were left
without any treatment, and BLS was allowed to develop (Xcv).
Lentinula edodes mycelia culture filtrate was applied in the same
way to tomato foliage either 24 h before (LemcfXcv) or 24 h after
(XcvLemcf) Xcv inoculation. Another set of three plants was treated
with 100 ppm streptomycin sulfate (positive control) 24 h after Xcv
inoculation. Three plants were left un-inoculated but sprayed with
sterile saline (un-inoculated control). The effect of Lemcf foliar
application in vitro was determined by applying Lemcf on uninoculated
plants (Lemcf). There were three BRs of Lemcf, each BR
was applied in duplicate, and the mean of two values was used. The
treated leaflets on every plant were counted, and the plants were
visually observed daily for BLS symptoms or plant response to Lemcf
application. The infected leaflets were enumerated 14 d after
treatment and converted to the percentage of treated leaflets (BLS
incidence). The experiment was repeated twice with three BRs in
each treatment group and control. The data from un-inoculated
controls were not included for statistical analysis. The treatment
means were separated by the least significant test at 5% probability.
According to the LSD test, there was no significant difference
among treatments in three trials; thus, the data were pooled and
presented as one trial.