The effect of modified conditions ofxylanase treatment on pentosan removal was evaluated. Firstly,the reaction medium i.e. Sodium phosphate buffer of pH 7 wasreplaced with distilled water while the other conditions were keptsame. Then the reaction time was reduced from 2 h to 1 h. At lastthe incubation temperature was also lowered from 60◦C to 50◦Cto reduce energy and costs requirements. In all the above cases,the effect on pentosan content was the sole criterion for modifica-tion of enzyme treatment conditions. One way analysis of variance(ANOVA) was then conducted for all the values of pentosan resultedfrom the modified conditions by using Origin version 6.1 to checkany significant difference at 95% confidence level