Before dying, gels were fixed
with 50% (v/v) methanol and 5% acetic acid for at least
3 h, then transferred into staining solution [0.12% (w/v)
Coomassie brilliant blue G-250, 10% (w/v) ammonium
sulfate, 10% (w/v) phosphoric acid, and 20% (v/v)
methanol] overnight with gentle shaking. After staining,