The aim of the study was to evaluate the in vitro effects of Bothrops bilineata crude venom
(BbV) on isolated human neutrophil function. We proved that BbV isn’t toxic towards
human neutrophils. During an incubation of human neutrophils with BbV hydrogen
peroxide was produced. Moreover, BbV was able to stimulate neutrophil release of
proinflammatory mediators such as IL-8 and IL-6 as well as PGE2 and NETs liberation.
There is no data in the literature showing the effect of BbV on the production of IL-6 and
IL-8 or NETs liberation by isolated human neutrophils. Taken together our results testify
that BbV triggers relevant proinflammatory events in human neutrophils.