The selectivity
of this medium was based on the activity of a-glucosidase which is
displayed by all strains of Cronobacter spp. The colonies suspected
to belong to Cronobacter spp. were streaked onto trypsin-soybean
agar TSA (Oxoid, Poland), on which majority of Cronobacter
strains produce a characteristic yellow pigment. The final confirmation
of bacteria affiliation to the Cronobacter spp.was carried out
using API 20 E tests (BioMerieux, Poland). In order to control the
correct evaluation of growth of the analyzed bacteria, parallel inoculations
of C. sakazakii ATCC 29544 strains were performed onto
HiCrome and TSA media