width were
reduced by increasing interval subculture until 8 weeks,
however the witches’ broom symptom seems not to be
correlated with phytoplasma concentration.
The maintainance of phytoplasma through tissue culture
of diseased key lime can present a useful way to
maintain living phytoplasma for studies of phytoplasmaplant
interactions toward the understanding of phytoplasma
pathogenicity and also to study possibility of
their elimination by cryotherapy, or other in vitro techniques.