Cultured NK-92 cells were washed in cold PBS with 2% FCS and
0.1% sodium azide and then stained with fluorescein isothiocyanate
(FITC)- or phycoerythrin (PE)-conjugated mouse antihumanmonoclonal
antibodies for surface staining including antiCD16/CD56(FITC/PE),
anti-CD25/CD69(FITC/PE), anti-CD3/CD95
(FITC/PE), anti-NKG2D(Cy2), and anti-NKp46(APC)、anti-NKp44
(FITC) and anti-NKp44(PE) (Beckman Inmumotech, China Branch,
China) for flow cytometric analysis