Live Asian hard clams (M. lusoria), 25–30 clams/kg, were purchased
from a local market along the coast of the Andaman Sea
in Kantang, Trang Province, Thailand, during October-December,
2012. They were transported in a sack to the laboratory within
3 h. Upon arrival, the clams were manually shucked by cutting
the adductor muscle with a knife. The clam juice was removed
and the edible portion, approximately 12.36% of total weight,
was collected. The edible portion was then dissected into 3 parts
including the foot, mantle and viscera constituting 36.07%,
45.45% and 18.48% of edible portion, respectively. All portions were
powderised using a blender (Phillips, Guangzhou, China) in the
presence of liquid nitrogen. All three samples were packed in a
polyethylene bag, sealed and stored at -20 C until use. The storage
time was not longer than 1 month.