2.7 Integrated use of the yeast and hot water
treatment
Mangoes, cv. Nam Dok Mai, were washed
in distilled water and left to dry. Cell suspension
of I. orientalis was prepared from 4-day-old PDA
culture at concentration of 108 cells/mL. The
mangoes were immersed in hot water (52°C) for 5
min then immersed in yeast cell suspension for 30
min. The mangoes in the control treatment, after
hot water treatment, were immersed in distilled
water instead. There were three replicates each
with fifteen fruits arranged in RCBD. The mangoes
were placed in plastic baskets and covered with
newspapers. After fifteen days of incubation at
room temperature, lesion development was assessed
using the same scores previously mentioned