2.5. Antibacterial activity
The residues of different solvents extraction were dissolved in dimethyl sulfoxide at different concentrations (25, 50, 100,250, 500 mg/mL). Muller Hinton agar (Himedia, Mumbai) was prepared by dissolving 15.2 g in 400 mL of distilled water. Then the medium was sterilized by autoclaving at 121 °C and at 15 lb for 15 min. After sterilization, the medium and sterile Petri plates were transferred into laminar air flow chamber.
Approximately 15-20 mL of the sterile medium was poured in each sterile Petri plates. The medium was allowed to
solidify in laminar flow and inoculated with the overnight
bacterial cultures. The sterile Whatman fitter paper No.
1 disc was prepared and discs were soaked in the plant
extracts and then placed over the inoculated plates. After
24 h of incubation at 37 °C, the zone of inhibition around the
disc was measured[7].