Cytologic Methods
ThinPrep Pap tests (TPPTs; Hologic, Marlborough,
MA) were prepared according to the manufacturer’s specifications
from PreservCyt samples using an automated processor
(ThinPrep 3000). Staining of the slides was done on
a Sakura Tissue-Tek Automated Slide Stainer (Sakura
Finetek USA, Torrance, CA) according to an US Food and
Drug Administration (FDA)-approved manufacturer’s
protocol. Location-guided computer-assisted screening of
TPPT slides was accomplished using the ThinPrep Imaging
System.6