A colorimetric method was used according to Shantha and
Decker (1994) with some modifications. The lipid fraction was
extracted by the Bligh and Dyer (1959) method using a methanol,
chloroform and water solution in the proportion of 40:20:16
(v:v:v). The organic phase (200 mL) was mixed with 10 mL of 95%
ethanol, aqueous ammonium thiocyanate solution (200 mL; 30%
w/v) and ferric chloride solution (200 mL; 0.4% w/v in HCl) during
15 s. The solution was incubated for 5 min at room temperature,
and the absorbance was measured at 500 nm (spectrophotometer
UNICAM UVeVis, Helios, Cambridge, UK). Iron (III) chloride solu-
tion used to construct the calibration curve [range of 5e200 mgofFe
(III)] was prepared according to Shantha and Decker (1994). Each
analysis was performed in subdued light and the peroxide value
was expressed as milliequivalents of peroxide per kilogram of
sample.
A colorimetric method was used according to Shantha and
Decker (1994) with some modifications. The lipid fraction was
extracted by the Bligh and Dyer (1959) method using a methanol,
chloroform and water solution in the proportion of 40:20:16
(v:v:v). The organic phase (200 mL) was mixed with 10 mL of 95%
ethanol, aqueous ammonium thiocyanate solution (200 mL; 30%
w/v) and ferric chloride solution (200 mL; 0.4% w/v in HCl) during
15 s. The solution was incubated for 5 min at room temperature,
and the absorbance was measured at 500 nm (spectrophotometer
UNICAM UVeVis, Helios, Cambridge, UK). Iron (III) chloride solu-
tion used to construct the calibration curve [range of 5e200 mgofFe
(III)] was prepared according to Shantha and Decker (1994). Each
analysis was performed in subdued light and the peroxide value
was expressed as milliequivalents of peroxide per kilogram of
sample.
การแปล กรุณารอสักครู่..
