Immunologic assays are based on antigen-antibody reactions. Latex Microparticles are coated with a specific antibody directed against the analyte (antigen) to be measured.
A beam of monochromatic light is then passed through the suspension of microlatex particles. When the wavelength of light is greater than the diameter of the particles in suspension, only a small amount of light will be absorbed by the particles.
When the latex microparticles coated with specific antibody come in contact with the antigen present in the solution, the antigen attaches to the antibody and forms bridges between the particles, causing them to agglutinate.
As the diameter of the agglutinates becomes larger and closer to the wavelength of the monochromatic light beam, the greater the amount of light that is absorbed.
The increase in light absorbance is proportional to the size of the agglutinates, which, in turn, is proportional to the antigen level present in the sample, which is read from a standard curve.
Immunologic assays are based on antigen-antibody reactions. Latex Microparticles are coated with a specific antibody directed against the analyte (antigen) to be measured. A beam of monochromatic light is then passed through the suspension of microlatex particles. When the wavelength of light is greater than the diameter of the particles in suspension, only a small amount of light will be absorbed by the particles. When the latex microparticles coated with specific antibody come in contact with the antigen present in the solution, the antigen attaches to the antibody and forms bridges between the particles, causing them to agglutinate. As the diameter of the agglutinates becomes larger and closer to the wavelength of the monochromatic light beam, the greater the amount of light that is absorbed. The increase in light absorbance is proportional to the size of the agglutinates, which, in turn, is proportional to the antigen level present in the sample, which is read from a standard curve.
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