The overnight cultures of test bacterial strains were prepared and aseptically spreaded over Muller Hinton Agar (MHA) plates using sterile cotton swabs. Thereafter wells of 6 mm diameter were made over MHA plates using sterile cork borer. The wells were then loaded with 100 ml of sample containing 300 mg of extract which was prepared using dimethyl sulfoxide (DMSO).
Streptomycin was used as a positive control (25 mg) and DMSO was used as a negative control.
The plates were then incubated at 37 1C for 24 h and the growth inhibitory activity in terms of zone of inhibition (mm) formed around each well was measured and recorded.
The assay was carried out in triplicate.