The nucleus and oocyte increase in diameter to
54.3 ± 10.5 mm and 195.7 ± 46.2 mm, respectively
(Fig. 2e). Acidophilic yolk granules appear from the
circumference of the cytoplasm. The yolk granules
do not occupy the inside of the cytoplasm yet.
Although the nucleus does not usually have an
affinity for dyes, it becomes basophilic during the
latter part of this stage. Follicle cell thickness is
4.1 ± 1.0 mm.