2.3. Total soluble solids and pH of brine
The pH values of fermented brine were carried out by a pH
meter (Crison Basic 20). Total soluble solid content was determined
using a refractometer (Atago, RX-5000, Italy) that was calibrated
with deionized water before samples were subjected to
analysis.
2.4. Total polyphenols of olives
Total polyphenols were extracted from olive flesh following the
method reported by Amiot, Fleuriette, and Macheix (1986) and
measured spectrophotometrically at 725 nm after reaction with
the Folin–Ciocalteu’s reagent, and expressed as mg kg1 of gallic
acid by means of a calibration plot, using pure gallic acid standard
at different concentrations.