In the ROIs within the DMN, significant differences in the strength of rs-FCs from the aMPFC seed were observed in the paracentral lobuli and middle frontal gyrus (MFG) between the two groups. A significant difference in rs-FCs from the PCC seed was observed in the MPFC between the groups. The mean correlation coefficient (Fisher z-transformed) for each group is shown in Figure 2 and Figure 3. The strengths of all of these rs-FCs were lower in the ASD group compared with the TD group. Individuals with ASD showed no significantly higher strength of rs-FCs from the seed regions compared with the TD group.