Table 2 Ingredientand nutrient composition of experimentaldiets.
Nominal crude protein level (%) 27 30 33 36
Ingredients (g 100 g−1 diet as fed) Salmon fish meal 4.00 4.00 4.00 4.00 Solvent extracted soybean meal 18.90 21.40 23.90 26.47 Lupins (de-hulled) 20.80 23.60 26.40 29.14 Waxy maize starch 30.67 29.07 27.59 19.96 Pregelatinised waxy maize starch 10.00 5.62 1.15 0.00 Wheat gluten meal 5.00 5.00 5.00 5.00 Casein 5.48 6.53 7.59 8.63 Diatomaceous earth 1.76 1.79 1.77 4.60 Salmon fish oil 1.22 0.84 0.46 0.10 EPA vitamin/mineral premix 0.20 0.20 0.20 0.20 Sodium alginate 0.30 0.30 0.30 0.30 Vitamin E 0.01 0.01 0.01 0.01 Calcium sulphate 0.43 0.36 0.30 0.22 Monosodium phosphate 0.72 0.68 0.65 0.61 Arginine 0.31 0.37 0.41 0.46 Threonine 0.20 0.23 0.27 0.30 Ingredient composition (g 100 g−1 diet as fed), analysed and (calculated) Moisture 10.35 10.48 10.61 10.30 Crude protein 27.00 31.10 34.30 37.30 Digestible protein (calculated)a 20.27 23.54 26.13 28.57 Lipid 3.60 3.60 3.70 3.50 Gross energy (MJ kg−1) 17.00 17.25 17.64 17.27 Digestible energy (MJ kg−1)a (calculated) 12.24 12.35 12.57 12.53 Ash 5.02 5.31 5.24 8.17 NFE (calculated) 64.38 59.99 56.76 51.03 Digestible CP:GE (g MJ−1)a 16.57 19.06 20.79 22.80 Calculated amino acids (g 100 g−1) Arginine 2.22 2.50 2.77 3.04 Histidine 0.72 0.80 0.89 0.97 Isoleucine 1.28 1.44 1.59 1.74 Leucine 2.10 2.35 2.59 2.83 Lysine 1.52 1.71 1.90 2.09 Methionine 0.46 0.51 0.57 0.62 Phenylalanine 1.31 1.46 1.61 1.76 Threonine 1.22 1.37 1.53 1.67 Tryptophan 0.30 0.34 0.37 0.41 Valine 1.42 1.59 1.75 1.92 a DigestibleproteinandenergyvaluesbasedondatareportedbyFlemingetal.(1998) and Vandepeer (2005); NFE = Nitrogen free extract = 100% − (protein % + lipid % + ash%); EPA.EyrePeninsula AquafeedPty Ltd.