The physical environment during in vitro culture did not affect the observed parameters (shoot number, shoot-cluster weight, and shoot height) during the proliferation stage.
Neither did an increase in hormonal concentrations nor environmental conditions have a significant effect on the chlorophyll a (chl a) content of the leaves. However, the two factors, hormone concentration and culture environment,affected chl b content. The higher BA concentrations resulted in significantly increased chl b in the leaves: 170 Ag g1 for
growth chamber and 281 Ag g1 for nethouse conditions,which was statistically different ( P0.05).
The carotenoid content of the cultures under nethouse conditions was significantly lower (F test at P0.05) than under growth chamber conditions (Table 2).