3.11. Effect of different surfactants
Xylanase production was enhanced by the addition of various
enhancers such as tween-80, Triton X-100 and sodium
dodecyl sulfate (SDS). Results in Fig. 11 indicated that tween-
80 enhanced the enzyme production (66.2 1.66 U/g) as
compared to control (45.5 1.33 U/g). Triton X-100
(59.6 1.38 U/g) and SDS (48.2 1.13 U/g) also enhanced the
xylanase production up to some extent. Highest total protein
(0.96 0.14 mg/ml) secretion was found in case of tween-80
supplementation to the medium. Kuhad, Manchanda, and
Singh (1998) optimized cultural conditions for xylanase production
by a hyperxylanolytic mutant strain (NTG-19) of F.
oxysporum in shake flask cultures. They reported that enzyme
production was also enhanced by supplementation of tween-Further experiments were performed to test the suitable
concentration of tween-80 supplementation to the medium.
0.1e1.0% tween-80 concentrations were tested, among all
these tested concentration 0.2% found to be better for
maximum synthesis of xylanase from T.viride-IR05 under solid
state fermentation as shown in Fig. 12. Increased concentration
of tween-80 beyond this resulted in decline in enzyme
synthesis. Total protein content of 0.91 0.21 mg/ml was also
noted at 0.2% tween-80 supplementation. Saleem, Akhtar, and
Jamil (2002) reported that supplementation of 0.2% concentration
of tween-80 had a positive effect on the production of
xylanase by Bacillus subtilis.
80 and olive oil to the medium. Liu et al. (1998) stated that