2.2.6. Effect on Urine Volume
Twenty five adult rats (180-200 gm) were divided into 5 groups. The 1st group was kept as a
control whereas the 2nd was orally given furosemide in a dose of 20 mg/kg b.wt. Rats of the 3rd 4th and
5
th groups were used for studying the effect of the tested extracts and the new compound on urine
volume in doses of 50 and 100 and 25 mg/kg b.wt respectively.
2.2.7. Effect on Liver and Kidney Functions
Mature rats of 150-180 gm were divided into 3 equal groups. The 1st group was left as a
control, while the 2nd and 3rd were orally given the plant extract in doses of 50 and 100 mg/kg b.wt. for
21 days. Blood samples were collected from the orbital plexus of each rat and sera were separated.
The sera were used to determine the activity of alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) [16]. Levels of total proteins [17], albumine [18], globulin, urea [19] and
creatinine were estimated [20].